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The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 \u00b5m mesh, and then, samples were stored in 96-% ethanol. In total, the dataset contains 101 samples, including 5 control samples and 96 samples from the lakes. Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna). 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